OSW7/YFR039C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 24 4.7%
Arg[R] 17 3.3%
Asn[N] 30 5.9%
Asp[D] 39 7.6%
Cys[C] 6 1.2%
Gln[Q] 20 3.9%
Glu[E] 53 10.4%
Gly[G] 17 3.3%
His[H] 12 2.4%
Ile[I] 33 6.5%
Leu[L] 54 10.6%
Lys[K] 36 7.1%
Met[M] 12 2.4%
Phe[F] 25 4.9%
Pro[P] 9 1.8%
Ser[S] 42 8.2%
Thr[T] 25 4.9%
Trp[W] 8 1.6%
Tyr[Y] 22 4.3%
Val[V] 26 5.1%
Total Length 510

Instability index
Instability index (II) 38.29
This classifies the protein as stable

Atomic composition
Carbon 2649
Hydrogen 4079
Nitrogen 679
Oxygen 834
Sulfur 18
Total number of atoms 8259
Formula = C2649H4079N679O834S18

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 77155 M-1 cm-1
assuming NO Cys residues appear as half cystines 76780 M-1 cm-1
assuming all Cys residues are reduced 76780 M-1 cm-1
assuming all pairs of Cys residues form cystines 77155 M-1 cm-1

Aliphatic index
Aliphatic index 86.02

Coding Region Translation Calculations
Codon Bias -0.033
Codon Adaptation Index 0.131
Frequency of Optimal Codons 0.404
Hydropathicity of Protein -0.483
Aromaticity Score 0.108