Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
5.0% |
| Arg[R] |
31 |
3.6% |
| Asn[N] |
73 |
8.6% |
| Asp[D] |
66 |
7.7% |
| Cys[C] |
8 |
0.9% |
| Gln[Q] |
33 |
3.9% |
| Glu[E] |
54 |
6.3% |
| Gly[G] |
39 |
4.6% |
| His[H] |
14 |
1.6% |
| Ile[I] |
52 |
6.1% |
| Leu[L] |
107 |
12.5% |
| Lys[K] |
79 |
9.3% |
| Met[M] |
22 |
2.6% |
| Phe[F] |
35 |
4.1% |
| Pro[P] |
22 |
2.6% |
| Ser[S] |
58 |
6.8% |
| Thr[T] |
50 |
5.9% |
| Trp[W] |
8 |
0.9% |
| Tyr[Y] |
19 |
2.2% |
| Val[V] |
40 |
4.7% |
| Total Length |
853 |
|
| Instability index (II) |
32.79 |
| This classifies the protein as stable |
|
| Carbon |
4288 |
| Hydrogen |
6869 |
| Nitrogen |
1167 |
| Oxygen |
1327 |
| Sulfur |
30 |
| Total number of atoms |
13681 |
| Formula = C4288H6869N1167O1327S30 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
72810 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
72310 M-1 cm-1 |
| assuming all Cys residues are reduced |
72310 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
72810 M-1 cm-1 |
|
|
| Codon Bias |
0.065 |
| Codon Adaptation Index |
0.157 |
| Frequency of Optimal Codons |
0.450 |
| Hydropathicity of Protein |
-0.471 |
| Aromaticity Score |
0.073 |
|
