Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
32 |
4.7% |
| Arg[R] |
34 |
5.0% |
| Asn[N] |
42 |
6.2% |
| Asp[D] |
39 |
5.8% |
| Cys[C] |
18 |
2.7% |
| Gln[Q] |
29 |
4.3% |
| Glu[E] |
38 |
5.6% |
| Gly[G] |
27 |
4.0% |
| His[H] |
18 |
2.7% |
| Ile[I] |
54 |
8.0% |
| Leu[L] |
56 |
8.3% |
| Lys[K] |
43 |
6.3% |
| Met[M] |
10 |
1.5% |
| Phe[F] |
27 |
4.0% |
| Pro[P] |
34 |
5.0% |
| Ser[S] |
71 |
10.5% |
| Thr[T] |
57 |
8.4% |
| Trp[W] |
4 |
0.6% |
| Tyr[Y] |
19 |
2.8% |
| Val[V] |
26 |
3.8% |
| Total Length |
678 |
|
| Instability index (II) |
42.05 |
| This classifies the protein as unstable |
|
| Carbon |
3342 |
| Hydrogen |
5308 |
| Nitrogen |
934 |
| Oxygen |
1051 |
| Sulfur |
28 |
| Total number of atoms |
10663 |
| Formula = C3342H5308N934O1051S28 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
51435 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
50310 M-1 cm-1 |
| assuming all Cys residues are reduced |
50310 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
51435 M-1 cm-1 |
|
|
| Codon Bias |
0.101 |
| Codon Adaptation Index |
0.119 |
| Frequency of Optimal Codons |
0.474 |
| Hydropathicity of Protein |
-0.478 |
| Aromaticity Score |
0.074 |
|
