Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
93 |
4.1% |
| Arg[R] |
106 |
4.7% |
| Asn[N] |
184 |
8.1% |
| Asp[D] |
133 |
5.8% |
| Cys[C] |
26 |
1.1% |
| Gln[Q] |
79 |
3.5% |
| Glu[E] |
130 |
5.7% |
| Gly[G] |
88 |
3.9% |
| His[H] |
60 |
2.6% |
| Ile[I] |
140 |
6.1% |
| Leu[L] |
190 |
8.3% |
| Lys[K] |
174 |
7.6% |
| Met[M] |
42 |
1.8% |
| Phe[F] |
83 |
3.6% |
| Pro[P] |
106 |
4.7% |
| Ser[S] |
279 |
12.2% |
| Thr[T] |
165 |
7.2% |
| Trp[W] |
18 |
0.8% |
| Tyr[Y] |
79 |
3.5% |
| Val[V] |
103 |
4.5% |
| Total Length |
2278 |
|
| Instability index (II) |
43.40 |
| This classifies the protein as unstable |
|
| Carbon |
11274 |
| Hydrogen |
17817 |
| Nitrogen |
3171 |
| Oxygen |
3591 |
| Sulfur |
68 |
| Total number of atoms |
35921 |
| Formula = C11274H17817N3171O3591S68 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
223835 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
222210 M-1 cm-1 |
| assuming all Cys residues are reduced |
216710 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
218335 M-1 cm-1 |
|
|
| Codon Bias |
0.000 |
| Codon Adaptation Index |
0.000 |
| Frequency of Optimal Codons |
0.000 |
| Hydropathicity of Protein |
0.000 |
| Aromaticity Score |
0.000 |
|
