Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
23 |
4.6% |
| Arg[R] |
21 |
4.2% |
| Asn[N] |
31 |
6.2% |
| Asp[D] |
26 |
5.2% |
| Cys[C] |
5 |
1.0% |
| Gln[Q] |
26 |
5.2% |
| Glu[E] |
46 |
9.2% |
| Gly[G] |
27 |
5.4% |
| His[H] |
7 |
1.4% |
| Ile[I] |
27 |
5.4% |
| Leu[L] |
42 |
8.4% |
| Lys[K] |
50 |
10.0% |
| Met[M] |
11 |
2.2% |
| Phe[F] |
26 |
5.2% |
| Pro[P] |
19 |
3.8% |
| Ser[S] |
39 |
7.8% |
| Thr[T] |
24 |
4.8% |
| Trp[W] |
3 |
0.6% |
| Tyr[Y] |
17 |
3.4% |
| Val[V] |
29 |
5.8% |
| Total Length |
499 |
|
| Instability index (II) |
45.80 |
| This classifies the protein as unstable |
|
| Carbon |
2536 |
| Hydrogen |
4002 |
| Nitrogen |
686 |
| Oxygen |
781 |
| Sulfur |
16 |
| Total number of atoms |
8021 |
| Formula = C2536H4002N686O781S16 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
42080 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
41830 M-1 cm-1 |
| assuming all Cys residues are reduced |
41830 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
42080 M-1 cm-1 |
|
|
| Codon Bias |
0.169 |
| Codon Adaptation Index |
0.208 |
| Frequency of Optimal Codons |
0.515 |
| Hydropathicity of Protein |
-0.655 |
| Aromaticity Score |
0.092 |
|
