Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
26 |
4.9% |
| Arg[R] |
29 |
5.4% |
| Asn[N] |
23 |
4.3% |
| Asp[D] |
39 |
7.3% |
| Cys[C] |
10 |
1.9% |
| Gln[Q] |
13 |
2.4% |
| Glu[E] |
44 |
8.2% |
| Gly[G] |
33 |
6.2% |
| His[H] |
17 |
3.2% |
| Ile[I] |
39 |
7.3% |
| Leu[L] |
47 |
8.8% |
| Lys[K] |
41 |
7.7% |
| Met[M] |
14 |
2.6% |
| Phe[F] |
30 |
5.6% |
| Pro[P] |
24 |
4.5% |
| Ser[S] |
29 |
5.4% |
| Thr[T] |
20 |
3.7% |
| Trp[W] |
4 |
0.7% |
| Tyr[Y] |
24 |
4.5% |
| Val[V] |
29 |
5.4% |
| Total Length |
535 |
|
| Instability index (II) |
38.86 |
| This classifies the protein as stable |
|
| Carbon |
2777 |
| Hydrogen |
4297 |
| Nitrogen |
737 |
| Oxygen |
811 |
| Sulfur |
24 |
| Total number of atoms |
8646 |
| Formula = C2777H4297N737O811S24 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
58385 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
57760 M-1 cm-1 |
| assuming all Cys residues are reduced |
57760 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
58385 M-1 cm-1 |
|
|
| Codon Bias |
0.178 |
| Codon Adaptation Index |
0.210 |
| Frequency of Optimal Codons |
0.518 |
| Hydropathicity of Protein |
-0.424 |
| Aromaticity Score |
0.108 |
|
