Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
45 |
5.4% |
| Arg[R] |
38 |
4.5% |
| Asn[N] |
67 |
8.0% |
| Asp[D] |
49 |
5.8% |
| Cys[C] |
5 |
0.6% |
| Gln[Q] |
33 |
3.9% |
| Glu[E] |
59 |
7.0% |
| Gly[G] |
32 |
3.8% |
| His[H] |
15 |
1.8% |
| Ile[I] |
69 |
8.2% |
| Leu[L] |
108 |
12.9% |
| Lys[K] |
65 |
7.7% |
| Met[M] |
15 |
1.8% |
| Phe[F] |
30 |
3.6% |
| Pro[P] |
23 |
2.7% |
| Ser[S] |
72 |
8.6% |
| Thr[T] |
36 |
4.3% |
| Trp[W] |
8 |
1.0% |
| Tyr[Y] |
34 |
4.1% |
| Val[V] |
36 |
4.3% |
| Total Length |
839 |
|
| Instability index (II) |
45.18 |
| This classifies the protein as unstable |
|
| Carbon |
4302 |
| Hydrogen |
6848 |
| Nitrogen |
1156 |
| Oxygen |
1298 |
| Sulfur |
20 |
| Total number of atoms |
13624 |
| Formula = C4302H6848N1156O1298S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
94910 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
94660 M-1 cm-1 |
| assuming all Cys residues are reduced |
94660 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
94910 M-1 cm-1 |
|
|
| Codon Bias |
0.090 |
| Codon Adaptation Index |
0.155 |
| Frequency of Optimal Codons |
0.462 |
| Hydropathicity of Protein |
-0.365 |
| Aromaticity Score |
0.086 |
|
