SMC1/YFL008W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 61 5.0%
Arg[R] 60 4.9%
Asn[N] 86 7.0%
Asp[D] 70 5.7%
Cys[C] 6 0.5%
Gln[Q] 62 5.1%
Glu[E] 135 11.0%
Gly[G] 49 4.0%
His[H] 18 1.5%
Ile[I] 94 7.7%
Leu[L] 131 10.7%
Lys[K] 134 10.9%
Met[M] 17 1.4%
Phe[F] 39 3.2%
Pro[P] 22 1.8%
Ser[S] 94 7.7%
Thr[T] 53 4.3%
Trp[W] 3 0.2%
Tyr[Y] 40 3.3%
Val[V] 51 4.2%
Total Length 1225

Instability index
Instability index (II) 44.00
This classifies the protein as unstable

Atomic composition
Carbon 6218
Hydrogen 10068
Nitrogen 1726
Oxygen 1971
Sulfur 23
Total number of atoms 20006
Formula = C6218H10068N1726O1971S23

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 76475 M-1 cm-1
assuming NO Cys residues appear as half cystines 76100 M-1 cm-1
assuming all Cys residues are reduced 76100 M-1 cm-1
assuming all pairs of Cys residues form cystines 76475 M-1 cm-1

Aliphatic index
Aliphatic index 88.69

Coding Region Translation Calculations
Codon Bias 0.097
Codon Adaptation Index 0.163
Frequency of Optimal Codons 0.476
Hydropathicity of Protein -0.740
Aromaticity Score 0.067