Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
47 |
6.0% |
| Arg[R] |
31 |
4.0% |
| Asn[N] |
59 |
7.6% |
| Asp[D] |
48 |
6.2% |
| Cys[C] |
8 |
1.0% |
| Gln[Q] |
15 |
1.9% |
| Glu[E] |
33 |
4.2% |
| Gly[G] |
47 |
6.0% |
| His[H] |
24 |
3.1% |
| Ile[I] |
54 |
6.9% |
| Leu[L] |
73 |
9.4% |
| Lys[K] |
46 |
5.9% |
| Met[M] |
11 |
1.4% |
| Phe[F] |
26 |
3.3% |
| Pro[P] |
28 |
3.6% |
| Ser[S] |
86 |
11.0% |
| Thr[T] |
55 |
7.1% |
| Trp[W] |
11 |
1.4% |
| Tyr[Y] |
26 |
3.3% |
| Val[V] |
51 |
6.5% |
| Total Length |
779 |
|
| Instability index (II) |
37.85 |
| This classifies the protein as stable |
|
| Carbon |
3812 |
| Hydrogen |
5999 |
| Nitrogen |
1051 |
| Oxygen |
1183 |
| Sulfur |
19 |
| Total number of atoms |
12064 |
| Formula = C3812H5999N1051O1183S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
99740 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
99240 M-1 cm-1 |
| assuming all Cys residues are reduced |
99240 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
99740 M-1 cm-1 |
|
|
| Codon Bias |
0.042 |
| Codon Adaptation Index |
0.139 |
| Frequency of Optimal Codons |
0.432 |
| Hydropathicity of Protein |
-0.282 |
| Aromaticity Score |
0.081 |
|
