HSP12/YFL014W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 13 11.9%
Arg[R] 3 2.8%
Asn[N] 3 2.8%
Asp[D] 11 10.1%
Cys[C] 0 0.0%
Gln[Q] 6 5.5%
Glu[E] 11 10.1%
Gly[G] 13 11.9%
His[H] 2 1.8%
Ile[I] 1 0.9%
Leu[L] 3 2.8%
Lys[K] 15 13.8%
Met[M] 2 1.8%
Phe[F] 2 1.8%
Pro[P] 3 2.8%
Ser[S] 8 7.3%
Thr[T] 2 1.8%
Trp[W] 0 0.0%
Tyr[Y] 4 3.7%
Val[V] 7 6.4%
Total Length 109

Instability index
Instability index (II) 26.71
This classifies the protein as stable

Atomic composition
Carbon 496
Hydrogen 788
Nitrogen 146
Oxygen 177
Sulfur 2
Total number of atoms 1609
Formula = C496H788N146O177S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 5960 M-1 cm-1

Aliphatic index
Aliphatic index 44.86

Coding Region Translation Calculations
Codon Bias 0.703
Codon Adaptation Index 0.639
Frequency of Optimal Codons 0.822
Hydropathicity of Protein -1.209
Aromaticity Score 0.055