LPD1/YFL018C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 45 9.0%
Arg[R] 17 3.4%
Asn[N] 25 5.0%
Asp[D] 24 4.8%
Cys[C] 5 1.0%
Gln[Q] 13 2.6%
Glu[E] 38 7.6%
Gly[G] 48 9.6%
His[H] 14 2.8%
Ile[I] 40 8.0%
Leu[L] 37 7.4%
Lys[K] 47 9.4%
Met[M] 9 1.8%
Phe[F] 17 3.4%
Pro[P] 15 3.0%
Ser[S] 24 4.8%
Thr[T] 27 5.4%
Trp[W] 1 0.2%
Tyr[Y] 10 2.0%
Val[V] 43 8.6%
Total Length 499

Instability index
Instability index (II) 32.79
This classifies the protein as stable

Atomic composition
Carbon 2396
Hydrogen 3884
Nitrogen 664
Oxygen 723
Sulfur 14
Total number of atoms 7681
Formula = C2396H3884N664O723S14

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 20650 M-1 cm-1
assuming NO Cys residues appear as half cystines 20400 M-1 cm-1
assuming all Cys residues are reduced 20400 M-1 cm-1
assuming all pairs of Cys residues form cystines 20650 M-1 cm-1

Aliphatic index
Aliphatic index 94.19

Coding Region Translation Calculations
Codon Bias 0.324
Codon Adaptation Index 0.253
Frequency of Optimal Codons 0.603
Hydropathicity of Protein -0.181
Aromaticity Score 0.056