PAU5/YFL020C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 21 17.2%
Arg[R] 4 3.3%
Asn[N] 3 2.5%
Asp[D] 5 4.1%
Cys[C] 0 0.0%
Gln[Q] 3 2.5%
Glu[E] 6 4.9%
Gly[G] 7 5.7%
His[H] 2 1.6%
Ile[I] 9 7.4%
Leu[L] 8 6.6%
Lys[K] 2 1.6%
Met[M] 2 1.6%
Phe[F] 3 2.5%
Pro[P] 5 4.1%
Ser[S] 11 9.0%
Thr[T] 12 9.8%
Trp[W] 1 0.8%
Tyr[Y] 7 5.7%
Val[V] 11 9.0%
Total Length 122

Instability index
Instability index (II) 18.17
This classifies the protein as stable

Atomic composition
Carbon 576
Hydrogen 901
Nitrogen 147
Oxygen 181
Sulfur 2
Total number of atoms 1807
Formula = C576H901N147O181S2

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 15930 M-1 cm-1

Aliphatic index
Aliphatic index 97.70

Coding Region Translation Calculations
Codon Bias 0.584
Codon Adaptation Index 0.508
Frequency of Optimal Codons 0.748
Hydropathicity of Protein 0.307
Aromaticity Score 0.090