STE2/YFL026W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 30 7.0%
Arg[R] 16 3.7%
Asn[N] 23 5.3%
Asp[D] 22 5.1%
Cys[C] 2 0.5%
Gln[Q] 14 3.2%
Glu[E] 12 2.8%
Gly[G] 18 4.2%
His[H] 4 0.9%
Ile[I] 33 7.7%
Leu[L] 44 10.2%
Lys[K] 17 3.9%
Met[M] 11 2.6%
Phe[F] 27 6.3%
Pro[P] 15 3.5%
Ser[S] 49 11.4%
Thr[T] 45 10.4%
Trp[W] 3 0.7%
Tyr[Y] 19 4.4%
Val[V] 27 6.3%
Total Length 431

Instability index
Instability index (II) 37.81
This classifies the protein as stable

Atomic composition
Carbon 2165
Hydrogen 3382
Nitrogen 544
Oxygen 650
Sulfur 13
Total number of atoms 6754
Formula = C2165H3382N544O650S13

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 44935 M-1 cm-1
assuming NO Cys residues appear as half cystines 44810 M-1 cm-1
assuming all Cys residues are reduced 44810 M-1 cm-1
assuming all pairs of Cys residues form cystines 44935 M-1 cm-1

Aliphatic index
Aliphatic index 94.80

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000