MOB2/YFL034C-B Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 17 5.9%
Arg[R] 11 3.8%
Asn[N] 21 7.3%
Asp[D] 9 3.1%
Cys[C] 0 0.0%
Gln[Q] 22 7.7%
Glu[E] 12 4.2%
Gly[G] 7 2.4%
His[H] 12 4.2%
Ile[I] 16 5.6%
Leu[L] 22 7.7%
Lys[K] 19 6.6%
Met[M] 7 2.4%
Phe[F] 24 8.4%
Pro[P] 19 6.6%
Ser[S] 28 9.8%
Thr[T] 14 4.9%
Trp[W] 4 1.4%
Tyr[Y] 9 3.1%
Val[V] 14 4.9%
Total Length 287

Instability index
Instability index (II) 48.21
This classifies the protein as unstable

Atomic composition
Carbon 1516
Hydrogen 2298
Nitrogen 410
Oxygen 424
Sulfur 7
Total number of atoms 4655
Formula = C1516H2298N410O424S7

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 35410 M-1 cm-1

Aliphatic index
Aliphatic index 71.71

Coding Region Translation Calculations
Codon Bias 0.017
Codon Adaptation Index 0.106
Frequency of Optimal Codons 0.424
Hydropathicity of Protein -0.492
Aromaticity Score 0.129