Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
73 |
5.4% |
| Arg[R] |
65 |
4.8% |
| Asn[N] |
80 |
5.9% |
| Asp[D] |
82 |
6.1% |
| Cys[C] |
8 |
0.6% |
| Gln[Q] |
49 |
3.6% |
| Glu[E] |
83 |
6.1% |
| Gly[G] |
62 |
4.6% |
| His[H] |
33 |
2.4% |
| Ile[I] |
79 |
5.8% |
| Leu[L] |
150 |
11.1% |
| Lys[K] |
116 |
8.6% |
| Met[M] |
28 |
2.1% |
| Phe[F] |
56 |
4.1% |
| Pro[P] |
66 |
4.9% |
| Ser[S] |
121 |
9.0% |
| Thr[T] |
66 |
4.9% |
| Trp[W] |
19 |
1.4% |
| Tyr[Y] |
31 |
2.3% |
| Val[V] |
84 |
6.2% |
| Total Length |
1351 |
|
| Instability index (II) |
42.29 |
| This classifies the protein as unstable |
|
| Carbon |
6842 |
| Hydrogen |
10888 |
| Nitrogen |
1876 |
| Oxygen |
2029 |
| Sulfur |
36 |
| Total number of atoms |
21671 |
| Formula = C6842H10888N1876O2029S36 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
151190 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
150690 M-1 cm-1 |
| assuming all Cys residues are reduced |
150690 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
151190 M-1 cm-1 |
|
|
| Codon Bias |
0.123 |
| Codon Adaptation Index |
0.169 |
| Frequency of Optimal Codons |
0.475 |
| Hydropathicity of Protein |
-0.422 |
| Aromaticity Score |
0.078 |
|
