Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
56 |
6.5% |
| Arg[R] |
57 |
6.6% |
| Asn[N] |
53 |
6.2% |
| Asp[D] |
50 |
5.8% |
| Cys[C] |
10 |
1.2% |
| Gln[Q] |
31 |
3.6% |
| Glu[E] |
59 |
6.9% |
| Gly[G] |
40 |
4.7% |
| His[H] |
21 |
2.4% |
| Ile[I] |
39 |
4.5% |
| Leu[L] |
66 |
7.7% |
| Lys[K] |
44 |
5.1% |
| Met[M] |
22 |
2.6% |
| Phe[F] |
36 |
4.2% |
| Pro[P] |
30 |
3.5% |
| Ser[S] |
104 |
12.1% |
| Thr[T] |
57 |
6.6% |
| Trp[W] |
7 |
0.8% |
| Tyr[Y] |
30 |
3.5% |
| Val[V] |
46 |
5.4% |
| Total Length |
858 |
|
| Instability index (II) |
40.88 |
| This classifies the protein as unstable |
|
| Carbon |
4203 |
| Hydrogen |
6600 |
| Nitrogen |
1206 |
| Oxygen |
1352 |
| Sulfur |
32 |
| Total number of atoms |
13393 |
| Formula = C4203H6600N1206O1352S32 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
83825 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
83200 M-1 cm-1 |
| assuming all Cys residues are reduced |
83200 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
83825 M-1 cm-1 |
|
|
| Codon Bias |
0.004 |
| Codon Adaptation Index |
0.122 |
| Frequency of Optimal Codons |
0.402 |
| Hydropathicity of Protein |
-0.601 |
| Aromaticity Score |
0.085 |
|
