Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
73 |
12.4% |
| Arg[R] |
18 |
3.0% |
| Asn[N] |
18 |
3.0% |
| Asp[D] |
33 |
5.6% |
| Cys[C] |
2 |
0.3% |
| Gln[Q] |
11 |
1.9% |
| Glu[E] |
38 |
6.4% |
| Gly[G] |
66 |
11.2% |
| His[H] |
12 |
2.0% |
| Ile[I] |
32 |
5.4% |
| Leu[L] |
55 |
9.3% |
| Lys[K] |
41 |
6.9% |
| Met[M] |
12 |
2.0% |
| Phe[F] |
13 |
2.2% |
| Pro[P] |
25 |
4.2% |
| Ser[S] |
41 |
6.9% |
| Thr[T] |
32 |
5.4% |
| Trp[W] |
3 |
0.5% |
| Tyr[Y] |
22 |
3.7% |
| Val[V] |
44 |
7.4% |
| Total Length |
591 |
|
| Instability index (II) |
31.51 |
| This classifies the protein as stable |
|
| Carbon |
2758 |
| Hydrogen |
4404 |
| Nitrogen |
742 |
| Oxygen |
858 |
| Sulfur |
14 |
| Total number of atoms |
8776 |
| Formula = C2758H4404N742O858S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
49405 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
49280 M-1 cm-1 |
| assuming all Cys residues are reduced |
49280 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
49405 M-1 cm-1 |
|
|
| Codon Bias |
0.166 |
| Codon Adaptation Index |
0.153 |
| Frequency of Optimal Codons |
0.486 |
| Hydropathicity of Protein |
-0.083 |
| Aromaticity Score |
0.064 |
|
