Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
56 |
10.0% |
| Arg[R] |
22 |
3.9% |
| Asn[N] |
22 |
3.9% |
| Asp[D] |
20 |
3.6% |
| Cys[C] |
5 |
0.9% |
| Gln[Q] |
8 |
1.4% |
| Glu[E] |
21 |
3.8% |
| Gly[G] |
49 |
8.8% |
| His[H] |
9 |
1.6% |
| Ile[I] |
50 |
9.0% |
| Leu[L] |
59 |
10.6% |
| Lys[K] |
19 |
3.4% |
| Met[M] |
6 |
1.1% |
| Phe[F] |
35 |
6.3% |
| Pro[P] |
24 |
4.3% |
| Ser[S] |
42 |
7.5% |
| Thr[T] |
25 |
4.5% |
| Trp[W] |
12 |
2.2% |
| Tyr[Y] |
27 |
4.8% |
| Val[V] |
47 |
8.4% |
| Total Length |
558 |
|
| Instability index (II) |
35.08 |
| This classifies the protein as stable |
|
| Carbon |
2849 |
| Hydrogen |
4359 |
| Nitrogen |
703 |
| Oxygen |
765 |
| Sulfur |
11 |
| Total number of atoms |
8687 |
| Formula = C2849H4359N703O765S11 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
106480 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
106230 M-1 cm-1 |
| assuming all Cys residues are reduced |
106230 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
106480 M-1 cm-1 |
|
|
| Codon Bias |
0.088 |
| Codon Adaptation Index |
0.122 |
| Frequency of Optimal Codons |
0.446 |
| Hydropathicity of Protein |
0.473 |
| Aromaticity Score |
0.133 |
|
