AAD16/YFL057C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 18 11.8%
Arg[R] 7 4.6%
Asn[N] 4 2.6%
Asp[D] 6 3.9%
Cys[C] 0 0.0%
Gln[Q] 5 3.3%
Glu[E] 13 8.6%
Gly[G] 12 7.9%
His[H] 3 2.0%
Ile[I] 13 8.6%
Leu[L] 9 5.9%
Lys[K] 13 8.6%
Met[M] 5 3.3%
Phe[F] 8 5.3%
Pro[P] 6 3.9%
Ser[S] 11 7.2%
Thr[T] 7 4.6%
Trp[W] 1 0.7%
Tyr[Y] 2 1.3%
Val[V] 9 5.9%
Total Length 152

Instability index
Instability index (II) 49.22
This classifies the protein as unstable

Atomic composition
Carbon 740
Hydrogen 1188
Nitrogen 202
Oxygen 220
Sulfur 5
Total number of atoms 2355
Formula = C740H1188N202O220S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 8480 M-1 cm-1

Aliphatic index
Aliphatic index 85.46

Coding Region Translation Calculations
Codon Bias 0.172
Codon Adaptation Index 0.169
Frequency of Optimal Codons 0.507
Hydropathicity of Protein -0.175
Aromaticity Score 0.072