YFL065C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 4 3.9%
Arg[R] 8 7.8%
Asn[N] 3 2.9%
Asp[D] 7 6.9%
Cys[C] 4 3.9%
Gln[Q] 3 2.9%
Glu[E] 4 3.9%
Gly[G] 4 3.9%
His[H] 2 2.0%
Ile[I] 6 5.9%
Leu[L] 11 10.8%
Lys[K] 9 8.8%
Met[M] 4 3.9%
Phe[F] 5 4.9%
Pro[P] 6 5.9%
Ser[S] 8 7.8%
Thr[T] 4 3.9%
Trp[W] 0 0.0%
Tyr[Y] 4 3.9%
Val[V] 6 5.9%
Total Length 102

Instability index
Instability index (II) 32.36
This classifies the protein as stable

Atomic composition
Carbon 524
Hydrogen 845
Nitrogen 145
Oxygen 147
Sulfur 8
Total number of atoms 1669
Formula = C524H845N145O147S8

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 6210 M-1 cm-1
assuming NO Cys residues appear as half cystines 5960 M-1 cm-1
assuming all Cys residues are reduced 5960 M-1 cm-1
assuming all pairs of Cys residues form cystines 6210 M-1 cm-1

Aliphatic index
Aliphatic index 85.98

Coding Region Translation Calculations
Codon Bias -0.048
Codon Adaptation Index 0.104
Frequency of Optimal Codons 0.367
Hydropathicity of Protein -0.292
Aromaticity Score 0.088