Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
72 |
6.2% |
| Arg[R] |
11 |
0.9% |
| Asn[N] |
45 |
3.8% |
| Asp[D] |
30 |
2.6% |
| Cys[C] |
35 |
3.0% |
| Gln[Q] |
18 |
1.5% |
| Glu[E] |
85 |
7.3% |
| Gly[G] |
38 |
3.3% |
| His[H] |
9 |
0.8% |
| Ile[I] |
42 |
3.6% |
| Leu[L] |
38 |
3.3% |
| Lys[K] |
31 |
2.7% |
| Met[M] |
13 |
1.1% |
| Phe[F] |
30 |
2.6% |
| Pro[P] |
49 |
4.2% |
| Ser[S] |
283 |
24.2% |
| Thr[T] |
194 |
16.6% |
| Trp[W] |
9 |
0.8% |
| Tyr[Y] |
47 |
4.0% |
| Val[V] |
90 |
7.7% |
| Total Length |
1169 |
|
| Instability index (II) |
58.60 |
| This classifies the protein as unstable |
|
| Carbon |
5175 |
| Hydrogen |
8083 |
| Nitrogen |
1323 |
| Oxygen |
1987 |
| Sulfur |
48 |
| Total number of atoms |
16616 |
| Formula = C5175H8083N1323O1987S48 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
121655 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
119530 M-1 cm-1 |
| assuming all Cys residues are reduced |
119530 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
121655 M-1 cm-1 |
|
|
| Codon Bias |
0.149 |
| Codon Adaptation Index |
0.162 |
| Frequency of Optimal Codons |
0.503 |
| Hydropathicity of Protein |
-0.265 |
| Aromaticity Score |
0.074 |
|
