Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
29 |
4.4% |
| Arg[R] |
39 |
6.0% |
| Asn[N] |
35 |
5.4% |
| Asp[D] |
40 |
6.1% |
| Cys[C] |
12 |
1.8% |
| Gln[Q] |
27 |
4.1% |
| Glu[E] |
47 |
7.2% |
| Gly[G] |
15 |
2.3% |
| His[H] |
9 |
1.4% |
| Ile[I] |
44 |
6.7% |
| Leu[L] |
77 |
11.8% |
| Lys[K] |
67 |
10.2% |
| Met[M] |
15 |
2.3% |
| Phe[F] |
29 |
4.4% |
| Pro[P] |
25 |
3.8% |
| Ser[S] |
38 |
5.8% |
| Thr[T] |
30 |
4.6% |
| Trp[W] |
8 |
1.2% |
| Tyr[Y] |
28 |
4.3% |
| Val[V] |
40 |
6.1% |
| Total Length |
654 |
|
| Instability index (II) |
42.29 |
| This classifies the protein as unstable |
|
| Carbon |
3474 |
| Hydrogen |
5536 |
| Nitrogen |
926 |
| Oxygen |
987 |
| Sulfur |
27 |
| Total number of atoms |
10950 |
| Formula = C3474H5536N926O987S27 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
86470 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
85720 M-1 cm-1 |
| assuming all Cys residues are reduced |
85720 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
86470 M-1 cm-1 |
|
|
| Codon Bias |
-0.072 |
| Codon Adaptation Index |
0.106 |
| Frequency of Optimal Codons |
0.371 |
| Hydropathicity of Protein |
-0.424 |
| Aromaticity Score |
0.099 |
|
