Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
7.8% |
| Arg[R] |
21 |
3.8% |
| Asn[N] |
27 |
4.9% |
| Asp[D] |
27 |
4.9% |
| Cys[C] |
10 |
1.8% |
| Gln[Q] |
15 |
2.7% |
| Glu[E] |
36 |
6.6% |
| Gly[G] |
36 |
6.6% |
| His[H] |
16 |
2.9% |
| Ile[I] |
36 |
6.6% |
| Leu[L] |
40 |
7.3% |
| Lys[K] |
32 |
5.8% |
| Met[M] |
14 |
2.6% |
| Phe[F] |
21 |
3.8% |
| Pro[P] |
31 |
5.6% |
| Ser[S] |
42 |
7.7% |
| Thr[T] |
39 |
7.1% |
| Trp[W] |
8 |
1.5% |
| Tyr[Y] |
22 |
4.0% |
| Val[V] |
33 |
6.0% |
| Total Length |
549 |
|
| Instability index (II) |
43.29 |
| This classifies the protein as unstable |
|
| Carbon |
2719 |
| Hydrogen |
4218 |
| Nitrogen |
726 |
| Oxygen |
821 |
| Sulfur |
24 |
| Total number of atoms |
8508 |
| Formula = C2719H4218N726O821S24 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
77405 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
76780 M-1 cm-1 |
| assuming all Cys residues are reduced |
76780 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
77405 M-1 cm-1 |
|
|
| Codon Bias |
0.379 |
| Codon Adaptation Index |
0.311 |
| Frequency of Optimal Codons |
0.632 |
| Hydropathicity of Protein |
-0.288 |
| Aromaticity Score |
0.093 |
|
