Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
45 |
5.1% |
| Arg[R] |
55 |
6.2% |
| Asn[N] |
34 |
3.8% |
| Asp[D] |
44 |
4.9% |
| Cys[C] |
11 |
1.2% |
| Gln[Q] |
36 |
4.0% |
| Glu[E] |
34 |
3.8% |
| Gly[G] |
46 |
5.2% |
| His[H] |
21 |
2.4% |
| Ile[I] |
56 |
6.3% |
| Leu[L] |
90 |
10.1% |
| Lys[K] |
56 |
6.3% |
| Met[M] |
23 |
2.6% |
| Phe[F] |
57 |
6.4% |
| Pro[P] |
45 |
5.1% |
| Ser[S] |
93 |
10.5% |
| Thr[T] |
54 |
6.1% |
| Trp[W] |
12 |
1.3% |
| Tyr[Y] |
28 |
3.1% |
| Val[V] |
49 |
5.5% |
| Total Length |
889 |
|
| Instability index (II) |
50.37 |
| This classifies the protein as unstable |
|
| Carbon |
4567 |
| Hydrogen |
7146 |
| Nitrogen |
1234 |
| Oxygen |
1291 |
| Sulfur |
34 |
| Total number of atoms |
14272 |
| Formula = C4567H7146N1234O1291S34 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
108345 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
107720 M-1 cm-1 |
| assuming all Cys residues are reduced |
107720 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
108345 M-1 cm-1 |
|
|
| Codon Bias |
0.131 |
| Codon Adaptation Index |
0.129 |
| Frequency of Optimal Codons |
0.475 |
| Hydropathicity of Protein |
-0.213 |
| Aromaticity Score |
0.109 |
|
