Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
156 |
6.3% |
| Arg[R] |
120 |
4.9% |
| Asn[N] |
134 |
5.4% |
| Asp[D] |
125 |
5.1% |
| Cys[C] |
31 |
1.3% |
| Gln[Q] |
110 |
4.4% |
| Glu[E] |
160 |
6.5% |
| Gly[G] |
99 |
4.0% |
| His[H] |
65 |
2.6% |
| Ile[I] |
185 |
7.5% |
| Leu[L] |
316 |
12.8% |
| Lys[K] |
163 |
6.6% |
| Met[M] |
58 |
2.3% |
| Phe[F] |
92 |
3.7% |
| Pro[P] |
101 |
4.1% |
| Ser[S] |
196 |
7.9% |
| Thr[T] |
119 |
4.8% |
| Trp[W] |
34 |
1.4% |
| Tyr[Y] |
74 |
3.0% |
| Val[V] |
136 |
5.5% |
| Total Length |
2474 |
|
| Instability index (II) |
45.62 |
| This classifies the protein as unstable |
|
| Carbon |
12646 |
| Hydrogen |
20125 |
| Nitrogen |
3405 |
| Oxygen |
3678 |
| Sulfur |
89 |
| Total number of atoms |
39943 |
| Formula = C12646H20125N3405O3678S89 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
299135 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
297260 M-1 cm-1 |
| assuming all Cys residues are reduced |
297260 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
299135 M-1 cm-1 |
|
|
| Codon Bias |
0.102 |
| Codon Adaptation Index |
0.152 |
| Frequency of Optimal Codons |
0.462 |
| Hydropathicity of Protein |
-0.191 |
| Aromaticity Score |
0.081 |
|
