Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
41 |
6.0% |
| Arg[R] |
31 |
4.6% |
| Asn[N] |
53 |
7.8% |
| Asp[D] |
33 |
4.9% |
| Cys[C] |
1 |
0.1% |
| Gln[Q] |
35 |
5.2% |
| Glu[E] |
79 |
11.6% |
| Gly[G] |
25 |
3.7% |
| His[H] |
9 |
1.3% |
| Ile[I] |
33 |
4.9% |
| Leu[L] |
75 |
11.0% |
| Lys[K] |
72 |
10.6% |
| Met[M] |
13 |
1.9% |
| Phe[F] |
13 |
1.9% |
| Pro[P] |
11 |
1.6% |
| Ser[S] |
66 |
9.7% |
| Thr[T] |
35 |
5.2% |
| Trp[W] |
3 |
0.4% |
| Tyr[Y] |
22 |
3.2% |
| Val[V] |
29 |
4.3% |
| Total Length |
679 |
|
| Instability index (II) |
44.01 |
| This classifies the protein as unstable |
|
| Carbon |
3361 |
| Hydrogen |
5465 |
| Nitrogen |
953 |
| Oxygen |
1115 |
| Sulfur |
14 |
| Total number of atoms |
10908 |
| Formula = C3361H5465N953O1115S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
49280 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
49280 M-1 cm-1 |
| assuming all Cys residues are reduced |
49280 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
49280 M-1 cm-1 |
|
|
| Codon Bias |
0.035 |
| Codon Adaptation Index |
0.126 |
| Frequency of Optimal Codons |
0.430 |
| Hydropathicity of Protein |
-0.873 |
| Aromaticity Score |
0.056 |
|
