Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
33 |
5.3% |
| Arg[R] |
15 |
2.4% |
| Asn[N] |
32 |
5.2% |
| Asp[D] |
27 |
4.4% |
| Cys[C] |
8 |
1.3% |
| Gln[Q] |
10 |
1.6% |
| Glu[E] |
32 |
5.2% |
| Gly[G] |
34 |
5.5% |
| His[H] |
13 |
2.1% |
| Ile[I] |
55 |
8.9% |
| Leu[L] |
78 |
12.6% |
| Lys[K] |
26 |
4.2% |
| Met[M] |
17 |
2.8% |
| Phe[F] |
44 |
7.1% |
| Pro[P] |
24 |
3.9% |
| Ser[S] |
54 |
8.7% |
| Thr[T] |
34 |
5.5% |
| Trp[W] |
9 |
1.5% |
| Tyr[Y] |
21 |
3.4% |
| Val[V] |
52 |
8.4% |
| Total Length |
618 |
|
| Instability index (II) |
36.30 |
| This classifies the protein as stable |
|
| Carbon |
3206 |
| Hydrogen |
4958 |
| Nitrogen |
766 |
| Oxygen |
888 |
| Sulfur |
25 |
| Total number of atoms |
9843 |
| Formula = C3206H4958N766O888S25 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
81290 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
80790 M-1 cm-1 |
| assuming all Cys residues are reduced |
80790 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
81290 M-1 cm-1 |
|
|
| Codon Bias |
0.007 |
| Codon Adaptation Index |
0.119 |
| Frequency of Optimal Codons |
0.414 |
| Hydropathicity of Protein |
0.451 |
| Aromaticity Score |
0.120 |
|
