Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
4.7% |
| Arg[R] |
30 |
3.3% |
| Asn[N] |
43 |
4.7% |
| Asp[D] |
43 |
4.7% |
| Cys[C] |
5 |
0.5% |
| Gln[Q] |
26 |
2.8% |
| Glu[E] |
39 |
4.2% |
| Gly[G] |
46 |
5.0% |
| His[H] |
26 |
2.8% |
| Ile[I] |
88 |
9.6% |
| Leu[L] |
108 |
11.8% |
| Lys[K] |
42 |
4.6% |
| Met[M] |
19 |
2.1% |
| Phe[F] |
77 |
8.4% |
| Pro[P] |
34 |
3.7% |
| Ser[S] |
78 |
8.5% |
| Thr[T] |
56 |
6.1% |
| Trp[W] |
17 |
1.8% |
| Tyr[Y] |
45 |
4.9% |
| Val[V] |
54 |
5.9% |
| Total Length |
919 |
|
| Instability index (II) |
44.42 |
| This classifies the protein as unstable |
|
| Carbon |
4947 |
| Hydrogen |
7481 |
| Nitrogen |
1189 |
| Oxygen |
1332 |
| Sulfur |
24 |
| Total number of atoms |
14973 |
| Formula = C4947H7481N1189O1332S24 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
160800 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
160550 M-1 cm-1 |
| assuming all Cys residues are reduced |
160550 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
160800 M-1 cm-1 |
|
|
| Codon Bias |
0.084 |
| Codon Adaptation Index |
0.159 |
| Frequency of Optimal Codons |
0.464 |
| Hydropathicity of Protein |
0.235 |
| Aromaticity Score |
0.151 |
|
