DBR1/YKL149C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 14 3.5%
Arg[R] 23 5.7%
Asn[N] 31 7.7%
Asp[D] 27 6.7%
Cys[C] 5 1.2%
Gln[Q] 15 3.7%
Glu[E] 26 6.4%
Gly[G] 20 4.9%
His[H] 16 4.0%
Ile[I] 37 9.1%
Leu[L] 35 8.6%
Lys[K] 31 7.7%
Met[M] 10 2.5%
Phe[F] 17 4.2%
Pro[P] 19 4.7%
Ser[S] 27 6.7%
Thr[T] 12 3.0%
Trp[W] 14 3.5%
Tyr[Y] 13 3.2%
Val[V] 13 3.2%
Total Length 405

Instability index
Instability index (II) 46.34
This classifies the protein as unstable

Atomic composition
Carbon 2149
Hydrogen 3301
Nitrogen 597
Oxygen 610
Sulfur 15
Total number of atoms 6672
Formula = C2149H3301N597O610S15

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 96620 M-1 cm-1
assuming NO Cys residues appear as half cystines 96370 M-1 cm-1
assuming all Cys residues are reduced 96370 M-1 cm-1
assuming all pairs of Cys residues form cystines 96620 M-1 cm-1

Aliphatic index
Aliphatic index 82.10

Coding Region Translation Calculations
Codon Bias -0.003
Codon Adaptation Index 0.140
Frequency of Optimal Codons 0.417
Hydropathicity of Protein -0.646
Aromaticity Score 0.109