Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
14 |
2.7% |
| Arg[R] |
28 |
5.3% |
| Asn[N] |
57 |
10.8% |
| Asp[D] |
23 |
4.4% |
| Cys[C] |
6 |
1.1% |
| Gln[Q] |
24 |
4.5% |
| Glu[E] |
28 |
5.3% |
| Gly[G] |
29 |
5.5% |
| His[H] |
11 |
2.1% |
| Ile[I] |
26 |
4.9% |
| Leu[L] |
46 |
8.7% |
| Lys[K] |
46 |
8.7% |
| Met[M] |
12 |
2.3% |
| Phe[F] |
17 |
3.2% |
| Pro[P] |
25 |
4.7% |
| Ser[S] |
51 |
9.7% |
| Thr[T] |
32 |
6.1% |
| Trp[W] |
4 |
0.8% |
| Tyr[Y] |
26 |
4.9% |
| Val[V] |
23 |
4.4% |
| Total Length |
528 |
|
| Instability index (II) |
43.08 |
| This classifies the protein as unstable |
|
| Carbon |
2652 |
| Hydrogen |
4187 |
| Nitrogen |
765 |
| Oxygen |
821 |
| Sulfur |
18 |
| Total number of atoms |
8443 |
| Formula = C2652H4187N765O821S18 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
61115 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
60740 M-1 cm-1 |
| assuming all Cys residues are reduced |
60740 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
61115 M-1 cm-1 |
|
|
| Codon Bias |
-0.004 |
| Codon Adaptation Index |
0.120 |
| Frequency of Optimal Codons |
0.408 |
| Hydropathicity of Protein |
-0.863 |
| Aromaticity Score |
0.089 |
|
