Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
27 |
4.3% |
| Arg[R] |
25 |
4.0% |
| Asn[N] |
34 |
5.4% |
| Asp[D] |
54 |
8.6% |
| Cys[C] |
10 |
1.6% |
| Gln[Q] |
29 |
4.6% |
| Glu[E] |
50 |
8.0% |
| Gly[G] |
24 |
3.8% |
| His[H] |
8 |
1.3% |
| Ile[I] |
51 |
8.1% |
| Leu[L] |
60 |
9.6% |
| Lys[K] |
38 |
6.1% |
| Met[M] |
16 |
2.6% |
| Phe[F] |
35 |
5.6% |
| Pro[P] |
17 |
2.7% |
| Ser[S] |
58 |
9.3% |
| Thr[T] |
29 |
4.6% |
| Trp[W] |
9 |
1.4% |
| Tyr[Y] |
24 |
3.8% |
| Val[V] |
29 |
4.6% |
| Total Length |
627 |
|
| Instability index (II) |
42.50 |
| This classifies the protein as unstable |
|
| Carbon |
3228 |
| Hydrogen |
4986 |
| Nitrogen |
828 |
| Oxygen |
1010 |
| Sulfur |
26 |
| Total number of atoms |
10078 |
| Formula = C3228H4986N828O1010S26 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
85885 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
85260 M-1 cm-1 |
| assuming all Cys residues are reduced |
85260 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
85885 M-1 cm-1 |
|
|
| Codon Bias |
0.016 |
| Codon Adaptation Index |
0.142 |
| Frequency of Optimal Codons |
0.432 |
| Hydropathicity of Protein |
-0.370 |
| Aromaticity Score |
0.108 |
|
