Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
31 |
3.6% |
| Arg[R] |
38 |
4.5% |
| Asn[N] |
65 |
7.6% |
| Asp[D] |
48 |
5.6% |
| Cys[C] |
18 |
2.1% |
| Gln[Q] |
22 |
2.6% |
| Glu[E] |
42 |
4.9% |
| Gly[G] |
46 |
5.4% |
| His[H] |
31 |
3.6% |
| Ile[I] |
51 |
6.0% |
| Leu[L] |
62 |
7.3% |
| Lys[K] |
62 |
7.3% |
| Met[M] |
14 |
1.6% |
| Phe[F] |
51 |
6.0% |
| Pro[P] |
37 |
4.3% |
| Ser[S] |
108 |
12.7% |
| Thr[T] |
56 |
6.6% |
| Trp[W] |
7 |
0.8% |
| Tyr[Y] |
26 |
3.1% |
| Val[V] |
37 |
4.3% |
| Total Length |
852 |
|
| Instability index (II) |
40.74 |
| This classifies the protein as unstable |
|
| Carbon |
4233 |
| Hydrogen |
6558 |
| Nitrogen |
1184 |
| Oxygen |
1310 |
| Sulfur |
32 |
| Total number of atoms |
13317 |
| Formula = C4233H6558N1184O1310S32 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
78365 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
77240 M-1 cm-1 |
| assuming all Cys residues are reduced |
77240 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
78365 M-1 cm-1 |
|
|
| Codon Bias |
0.018 |
| Codon Adaptation Index |
0.136 |
| Frequency of Optimal Codons |
0.425 |
| Hydropathicity of Protein |
-0.568 |
| Aromaticity Score |
0.099 |
|
