Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
40 |
4.2% |
| Arg[R] |
14 |
1.5% |
| Asn[N] |
144 |
15.0% |
| Asp[D] |
24 |
2.5% |
| Cys[C] |
5 |
0.5% |
| Gln[Q] |
61 |
6.4% |
| Glu[E] |
19 |
2.0% |
| Gly[G] |
120 |
12.5% |
| His[H] |
7 |
0.7% |
| Ile[I] |
27 |
2.8% |
| Leu[L] |
67 |
7.0% |
| Lys[K] |
45 |
4.7% |
| Met[M] |
10 |
1.0% |
| Phe[F] |
60 |
6.3% |
| Pro[P] |
45 |
4.7% |
| Ser[S] |
138 |
14.4% |
| Thr[T] |
92 |
9.6% |
| Trp[W] |
1 |
0.1% |
| Tyr[Y] |
12 |
1.3% |
| Val[V] |
28 |
2.9% |
| Total Length |
959 |
|
| Instability index (II) |
29.62 |
| This classifies the protein as stable |
|
| Carbon |
4263 |
| Hydrogen |
6632 |
| Nitrogen |
1266 |
| Oxygen |
1493 |
| Sulfur |
15 |
| Total number of atoms |
13669 |
| Formula = C4263H6632N1266O1493S15 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
23630 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
23380 M-1 cm-1 |
| assuming all Cys residues are reduced |
23380 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
23630 M-1 cm-1 |
|
|
| Codon Bias |
-0.009 |
| Codon Adaptation Index |
0.107 |
| Frequency of Optimal Codons |
0.392 |
| Hydropathicity of Protein |
-0.704 |
| Aromaticity Score |
0.076 |
|
