TMA19/YKL056C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 13 7.8%
Arg[R] 2 1.2%
Asn[N] 8 4.8%
Asp[D] 17 10.2%
Cys[C] 1 0.6%
Gln[Q] 4 2.4%
Glu[E] 15 9.0%
Gly[G] 12 7.2%
His[H] 2 1.2%
Ile[I] 11 6.6%
Leu[L] 7 4.2%
Lys[K] 15 9.0%
Met[M] 7 4.2%
Phe[F] 9 5.4%
Pro[P] 5 3.0%
Ser[S] 7 4.2%
Thr[T] 7 4.2%
Trp[W] 2 1.2%
Tyr[Y] 7 4.2%
Val[V] 16 9.6%
Total Length 167

Instability index
Instability index (II) 22.18
This classifies the protein as stable

Atomic composition
Carbon 838
Hydrogen 1284
Nitrogen 206
Oxygen 265
Sulfur 8
Total number of atoms 2601
Formula = C838H1284N206O265S8

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 21430 M-1 cm-1
assuming NO Cys residues appear as half cystines 21430 M-1 cm-1
assuming all Cys residues are reduced 21430 M-1 cm-1
assuming all pairs of Cys residues form cystines 21430 M-1 cm-1

Aliphatic index
Aliphatic index 77.60

Coding Region Translation Calculations
Codon Bias 0.730
Codon Adaptation Index 0.731
Frequency of Optimal Codons 0.848
Hydropathicity of Protein -0.326
Aromaticity Score 0.108