Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
23 |
4.6% |
| Arg[R] |
26 |
5.2% |
| Asn[N] |
33 |
6.6% |
| Asp[D] |
31 |
6.2% |
| Cys[C] |
4 |
0.8% |
| Gln[Q] |
12 |
2.4% |
| Glu[E] |
31 |
6.2% |
| Gly[G] |
30 |
6.0% |
| His[H] |
16 |
3.2% |
| Ile[I] |
34 |
6.8% |
| Leu[L] |
57 |
11.4% |
| Lys[K] |
35 |
7.0% |
| Met[M] |
7 |
1.4% |
| Phe[F] |
21 |
4.2% |
| Pro[P] |
21 |
4.2% |
| Ser[S] |
39 |
7.8% |
| Thr[T] |
29 |
5.8% |
| Trp[W] |
3 |
0.6% |
| Tyr[Y] |
11 |
2.2% |
| Val[V] |
36 |
7.2% |
| Total Length |
499 |
|
| Instability index (II) |
31.06 |
| This classifies the protein as stable |
|
| Carbon |
2494 |
| Hydrogen |
3988 |
| Nitrogen |
692 |
| Oxygen |
748 |
| Sulfur |
11 |
| Total number of atoms |
7933 |
| Formula = C2494H3988N692O748S11 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
33140 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
32890 M-1 cm-1 |
| assuming all Cys residues are reduced |
32890 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
33140 M-1 cm-1 |
|
|
| Codon Bias |
0.410 |
| Codon Adaptation Index |
0.330 |
| Frequency of Optimal Codons |
0.648 |
| Hydropathicity of Protein |
-0.299 |
| Aromaticity Score |
0.070 |
|
