Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
39 |
3.8% |
| Arg[R] |
37 |
3.6% |
| Asn[N] |
48 |
4.6% |
| Asp[D] |
61 |
5.9% |
| Cys[C] |
22 |
2.1% |
| Gln[Q] |
45 |
4.3% |
| Glu[E] |
89 |
8.6% |
| Gly[G] |
25 |
2.4% |
| His[H] |
22 |
2.1% |
| Ile[I] |
90 |
8.7% |
| Leu[L] |
129 |
12.4% |
| Lys[K] |
68 |
6.6% |
| Met[M] |
22 |
2.1% |
| Phe[F] |
49 |
4.7% |
| Pro[P] |
35 |
3.4% |
| Ser[S] |
100 |
9.6% |
| Thr[T] |
49 |
4.7% |
| Trp[W] |
8 |
0.8% |
| Tyr[Y] |
28 |
2.7% |
| Val[V] |
72 |
6.9% |
| Total Length |
1038 |
|
| Instability index (II) |
43.45 |
| This classifies the protein as unstable |
|
| Carbon |
5337 |
| Hydrogen |
8488 |
| Nitrogen |
1362 |
| Oxygen |
1609 |
| Sulfur |
44 |
| Total number of atoms |
16840 |
| Formula = C5337H8488N1362O1609S44 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
87095 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
85720 M-1 cm-1 |
| assuming all Cys residues are reduced |
85720 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
87095 M-1 cm-1 |
|
|
| Codon Bias |
-0.006 |
| Codon Adaptation Index |
0.125 |
| Frequency of Optimal Codons |
0.416 |
| Hydropathicity of Protein |
-0.072 |
| Aromaticity Score |
0.082 |
|
