Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
63 |
7.5% |
| Arg[R] |
31 |
3.7% |
| Asn[N] |
78 |
9.3% |
| Asp[D] |
55 |
6.5% |
| Cys[C] |
16 |
1.9% |
| Gln[Q] |
30 |
3.6% |
| Glu[E] |
44 |
5.2% |
| Gly[G] |
27 |
3.2% |
| His[H] |
17 |
2.0% |
| Ile[I] |
36 |
4.3% |
| Leu[L] |
93 |
11.1% |
| Lys[K] |
62 |
7.4% |
| Met[M] |
22 |
2.6% |
| Phe[F] |
34 |
4.0% |
| Pro[P] |
27 |
3.2% |
| Ser[S] |
77 |
9.2% |
| Thr[T] |
50 |
6.0% |
| Trp[W] |
6 |
0.7% |
| Tyr[Y] |
36 |
4.3% |
| Val[V] |
36 |
4.3% |
| Total Length |
840 |
|
| Instability index (II) |
38.53 |
| This classifies the protein as stable |
|
| Carbon |
4179 |
| Hydrogen |
6559 |
| Nitrogen |
1143 |
| Oxygen |
1310 |
| Sulfur |
38 |
| Total number of atoms |
13229 |
| Formula = C4179H6559N1143O1310S38 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
87640 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
86640 M-1 cm-1 |
| assuming all Cys residues are reduced |
86640 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
87640 M-1 cm-1 |
|
|
| Codon Bias |
-0.005 |
| Codon Adaptation Index |
0.124 |
| Frequency of Optimal Codons |
0.401 |
| Hydropathicity of Protein |
-0.483 |
| Aromaticity Score |
0.090 |
|
