UFD4/YKL010C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 66 4.5%
Arg[R] 71 4.8%
Asn[N] 73 4.9%
Asp[D] 112 7.6%
Cys[C] 23 1.6%
Gln[Q] 38 2.6%
Glu[E] 128 8.6%
Gly[G] 78 5.3%
His[H] 34 2.3%
Ile[I] 110 7.4%
Leu[L] 167 11.3%
Lys[K] 79 5.3%
Met[M] 33 2.2%
Phe[F] 77 5.2%
Pro[P] 44 3.0%
Ser[S] 139 9.4%
Thr[T] 78 5.3%
Trp[W] 7 0.5%
Tyr[Y] 43 2.9%
Val[V] 83 5.6%
Total Length 1483

Instability index
Instability index (II) 41.16
This classifies the protein as unstable

Atomic composition
Carbon 7445
Hydrogen 11707
Nitrogen 1961
Oxygen 2335
Sulfur 56
Total number of atoms 23504
Formula = C7445H11707N1961O2335S56

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 103945 M-1 cm-1
assuming NO Cys residues appear as half cystines 102570 M-1 cm-1
assuming all Cys residues are reduced 102570 M-1 cm-1
assuming all pairs of Cys residues form cystines 103945 M-1 cm-1

Aliphatic index
Aliphatic index 93.53

Coding Region Translation Calculations
Codon Bias 0.055
Codon Adaptation Index 0.139
Frequency of Optimal Codons 0.441
Hydropathicity of Protein -0.244
Aromaticity Score 0.086