Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
36 |
3.2% |
| Arg[R] |
42 |
3.8% |
| Asn[N] |
123 |
11.1% |
| Asp[D] |
71 |
6.4% |
| Cys[C] |
14 |
1.3% |
| Gln[Q] |
52 |
4.7% |
| Glu[E] |
84 |
7.5% |
| Gly[G] |
40 |
3.6% |
| His[H] |
25 |
2.2% |
| Ile[I] |
69 |
6.2% |
| Leu[L] |
109 |
9.8% |
| Lys[K] |
66 |
5.9% |
| Met[M] |
24 |
2.2% |
| Phe[F] |
53 |
4.8% |
| Pro[P] |
43 |
3.9% |
| Ser[S] |
109 |
9.8% |
| Thr[T] |
67 |
6.0% |
| Trp[W] |
7 |
0.6% |
| Tyr[Y] |
30 |
2.7% |
| Val[V] |
49 |
4.4% |
| Total Length |
1113 |
|
| Instability index (II) |
51.36 |
| This classifies the protein as unstable |
|
| Carbon |
5551 |
| Hydrogen |
8687 |
| Nitrogen |
1537 |
| Oxygen |
1805 |
| Sulfur |
38 |
| Total number of atoms |
17618 |
| Formula = C5551H8687N1537O1805S38 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
84075 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
83200 M-1 cm-1 |
| assuming all Cys residues are reduced |
83200 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
84075 M-1 cm-1 |
|
|
| Codon Bias |
0.060 |
| Codon Adaptation Index |
0.150 |
| Frequency of Optimal Codons |
0.455 |
| Hydropathicity of Protein |
-0.648 |
| Aromaticity Score |
0.081 |
|
