Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
16 |
4.3% |
| Arg[R] |
33 |
9.0% |
| Asn[N] |
28 |
7.6% |
| Asp[D] |
39 |
10.6% |
| Cys[C] |
0 |
0.0% |
| Gln[Q] |
15 |
4.1% |
| Glu[E] |
41 |
11.1% |
| Gly[G] |
20 |
5.4% |
| His[H] |
9 |
2.4% |
| Ile[I] |
18 |
4.9% |
| Leu[L] |
27 |
7.3% |
| Lys[K] |
14 |
3.8% |
| Met[M] |
10 |
2.7% |
| Phe[F] |
10 |
2.7% |
| Pro[P] |
15 |
4.1% |
| Ser[S] |
30 |
8.2% |
| Thr[T] |
9 |
2.4% |
| Trp[W] |
3 |
0.8% |
| Tyr[Y] |
15 |
4.1% |
| Val[V] |
16 |
4.3% |
| Total Length |
368 |
|
| Instability index (II) |
47.63 |
| This classifies the protein as unstable |
|
| Carbon |
1831 |
| Hydrogen |
2841 |
| Nitrogen |
545 |
| Oxygen |
626 |
| Sulfur |
10 |
| Total number of atoms |
5853 |
| Formula = C1831H2841N545O626S10 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
38850 M-1 cm-1 |
|
|
| Codon Bias |
-0.084 |
| Codon Adaptation Index |
0.096 |
| Frequency of Optimal Codons |
0.352 |
| Hydropathicity of Protein |
-1.142 |
| Aromaticity Score |
0.076 |
|
