SEC11/YIR022W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 12 7.2%
Arg[R] 5 3.0%
Asn[N] 14 8.4%
Asp[D] 5 3.0%
Cys[C] 1 0.6%
Gln[Q] 8 4.8%
Glu[E] 7 4.2%
Gly[G] 14 8.4%
His[H] 3 1.8%
Ile[I] 11 6.6%
Leu[L] 21 12.6%
Lys[K] 11 6.6%
Met[M] 4 2.4%
Phe[F] 10 6.0%
Pro[P] 4 2.4%
Ser[S] 9 5.4%
Thr[T] 5 3.0%
Trp[W] 3 1.8%
Tyr[Y] 7 4.2%
Val[V] 13 7.8%
Total Length 167

Instability index
Instability index (II) 31.30
This classifies the protein as stable

Atomic composition
Carbon 862
Hydrogen 1340
Nitrogen 224
Oxygen 235
Sulfur 5
Total number of atoms 2666
Formula = C862H1340N224O235S5

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 26930 M-1 cm-1
assuming NO Cys residues appear as half cystines 26930 M-1 cm-1
assuming all Cys residues are reduced 26930 M-1 cm-1
assuming all pairs of Cys residues form cystines 26930 M-1 cm-1

Aliphatic index
Aliphatic index 104.49

Coding Region Translation Calculations
Codon Bias 0.165
Codon Adaptation Index 0.165
Frequency of Optimal Codons 0.500
Hydropathicity of Protein 0.090
Aromaticity Score 0.120