Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
87 |
6.4% |
| Arg[R] |
2 |
0.1% |
| Asn[N] |
36 |
2.6% |
| Asp[D] |
14 |
1.0% |
| Cys[C] |
20 |
1.5% |
| Gln[Q] |
20 |
1.5% |
| Glu[E] |
69 |
5.0% |
| Gly[G] |
45 |
3.3% |
| His[H] |
5 |
0.4% |
| Ile[I] |
32 |
2.3% |
| Leu[L] |
29 |
2.1% |
| Lys[K] |
30 |
2.2% |
| Met[M] |
5 |
0.4% |
| Phe[F] |
18 |
1.3% |
| Pro[P] |
143 |
10.5% |
| Ser[S] |
347 |
25.4% |
| Thr[T] |
338 |
24.7% |
| Trp[W] |
8 |
0.6% |
| Tyr[Y] |
23 |
1.7% |
| Val[V] |
96 |
7.0% |
| Total Length |
1367 |
|
| Instability index (II) |
73.94 |
| This classifies the protein as unstable |
|
| Carbon |
5714 |
| Hydrogen |
9151 |
| Nitrogen |
1477 |
| Oxygen |
2298 |
| Sulfur |
25 |
| Total number of atoms |
18665 |
| Formula = C5714H9151N1477O2298S25 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
79520 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
78270 M-1 cm-1 |
| assuming all Cys residues are reduced |
78270 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
79520 M-1 cm-1 |
|
|
| Codon Bias |
0.492 |
| Codon Adaptation Index |
0.299 |
| Frequency of Optimal Codons |
0.696 |
| Hydropathicity of Protein |
-0.368 |
| Aromaticity Score |
0.036 |
|
