Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
49 |
4.9% |
| Arg[R] |
50 |
5.0% |
| Asn[N] |
64 |
6.4% |
| Asp[D] |
68 |
6.8% |
| Cys[C] |
17 |
1.7% |
| Gln[Q] |
42 |
4.2% |
| Glu[E] |
82 |
8.3% |
| Gly[G] |
41 |
4.1% |
| His[H] |
17 |
1.7% |
| Ile[I] |
78 |
7.9% |
| Leu[L] |
71 |
7.2% |
| Lys[K] |
99 |
10.0% |
| Met[M] |
21 |
2.1% |
| Phe[F] |
46 |
4.6% |
| Pro[P] |
33 |
3.3% |
| Ser[S] |
62 |
6.2% |
| Thr[T] |
56 |
5.6% |
| Trp[W] |
4 |
0.4% |
| Tyr[Y] |
30 |
3.0% |
| Val[V] |
63 |
6.3% |
| Total Length |
993 |
|
| Instability index (II) |
39.41 |
| This classifies the protein as stable |
|
| Carbon |
5041 |
| Hydrogen |
8048 |
| Nitrogen |
1386 |
| Oxygen |
1548 |
| Sulfur |
38 |
| Total number of atoms |
16061 |
| Formula = C5041H8048N1386O1548S38 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
67700 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
66700 M-1 cm-1 |
| assuming all Cys residues are reduced |
66700 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
67700 M-1 cm-1 |
|
|
| Codon Bias |
0.042 |
| Codon Adaptation Index |
0.152 |
| Frequency of Optimal Codons |
0.454 |
| Hydropathicity of Protein |
-0.581 |
| Aromaticity Score |
0.081 |
|
