YIL165C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 11 9.2%
Arg[R] 5 4.2%
Asn[N] 5 4.2%
Asp[D] 10 8.4%
Cys[C] 1 0.8%
Gln[Q] 4 3.4%
Glu[E] 7 5.9%
Gly[G] 13 10.9%
His[H] 2 1.7%
Ile[I] 11 9.2%
Leu[L] 10 8.4%
Lys[K] 5 4.2%
Met[M] 3 2.5%
Phe[F] 6 5.0%
Pro[P] 6 5.0%
Ser[S] 4 3.4%
Thr[T] 6 5.0%
Trp[W] 1 0.8%
Tyr[Y] 3 2.5%
Val[V] 6 5.0%
Total Length 119

Instability index
Instability index (II) 33.23
This classifies the protein as stable

Atomic composition
Carbon 578
Hydrogen 903
Nitrogen 153
Oxygen 176
Sulfur 4
Total number of atoms 1814
Formula = C578H903N153O176S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 9970 M-1 cm-1
assuming NO Cys residues appear as half cystines 9970 M-1 cm-1
assuming all Cys residues are reduced 9970 M-1 cm-1
assuming all pairs of Cys residues form cystines 9970 M-1 cm-1

Aliphatic index
Aliphatic index 92.69

Coding Region Translation Calculations
Codon Bias 0.143
Codon Adaptation Index 0.147
Frequency of Optimal Codons 0.487
Hydropathicity of Protein -0.075
Aromaticity Score 0.084