Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
15 |
2.2% |
| Arg[R] |
33 |
4.8% |
| Asn[N] |
57 |
8.2% |
| Asp[D] |
33 |
4.8% |
| Cys[C] |
2 |
0.3% |
| Gln[Q] |
43 |
6.2% |
| Glu[E] |
67 |
9.7% |
| Gly[G] |
24 |
3.5% |
| His[H] |
16 |
2.3% |
| Ile[I] |
62 |
9.0% |
| Leu[L] |
77 |
11.1% |
| Lys[K] |
73 |
10.6% |
| Met[M] |
13 |
1.9% |
| Phe[F] |
20 |
2.9% |
| Pro[P] |
13 |
1.9% |
| Ser[S] |
63 |
9.1% |
| Thr[T] |
40 |
5.8% |
| Trp[W] |
5 |
0.7% |
| Tyr[Y] |
17 |
2.5% |
| Val[V] |
18 |
2.6% |
| Total Length |
691 |
|
| Instability index (II) |
47.27 |
| This classifies the protein as unstable |
|
| Carbon |
3532 |
| Hydrogen |
5740 |
| Nitrogen |
1000 |
| Oxygen |
1112 |
| Sulfur |
15 |
| Total number of atoms |
11399 |
| Formula = C3532H5740N1000O1112S15 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
52955 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
52830 M-1 cm-1 |
| assuming all Cys residues are reduced |
52830 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
52955 M-1 cm-1 |
|
|
| Codon Bias |
0.019 |
| Codon Adaptation Index |
0.142 |
| Frequency of Optimal Codons |
0.437 |
| Hydropathicity of Protein |
-0.810 |
| Aromaticity Score |
0.061 |
|
