Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
138 |
5.8% |
| Arg[R] |
98 |
4.1% |
| Asn[N] |
145 |
6.1% |
| Asp[D] |
125 |
5.3% |
| Cys[C] |
30 |
1.3% |
| Gln[Q] |
84 |
3.5% |
| Glu[E] |
158 |
6.6% |
| Gly[G] |
80 |
3.4% |
| His[H] |
46 |
1.9% |
| Ile[I] |
173 |
7.3% |
| Leu[L] |
296 |
12.5% |
| Lys[K] |
155 |
6.5% |
| Met[M] |
58 |
2.4% |
| Phe[F] |
122 |
5.1% |
| Pro[P] |
93 |
3.9% |
| Ser[S] |
231 |
9.7% |
| Thr[T] |
134 |
5.6% |
| Trp[W] |
30 |
1.3% |
| Tyr[Y] |
63 |
2.7% |
| Val[V] |
117 |
4.9% |
| Total Length |
2376 |
|
| Instability index (II) |
44.13 |
| This classifies the protein as unstable |
|
| Carbon |
12126 |
| Hydrogen |
19156 |
| Nitrogen |
3176 |
| Oxygen |
3600 |
| Sulfur |
88 |
| Total number of atoms |
38146 |
| Formula = C12126H19156N3176O3600S88 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
260745 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
258870 M-1 cm-1 |
| assuming all Cys residues are reduced |
258870 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
260745 M-1 cm-1 |
|
|
| Codon Bias |
0.039 |
| Codon Adaptation Index |
0.128 |
| Frequency of Optimal Codons |
0.427 |
| Hydropathicity of Protein |
-0.161 |
| Aromaticity Score |
0.090 |
|
