Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
63 |
6.2% |
| Arg[R] |
49 |
4.8% |
| Asn[N] |
41 |
4.0% |
| Asp[D] |
53 |
5.2% |
| Cys[C] |
7 |
0.7% |
| Gln[Q] |
46 |
4.5% |
| Glu[E] |
71 |
7.0% |
| Gly[G] |
70 |
6.9% |
| His[H] |
34 |
3.4% |
| Ile[I] |
46 |
4.5% |
| Leu[L] |
98 |
9.7% |
| Lys[K] |
70 |
6.9% |
| Met[M] |
12 |
1.2% |
| Phe[F] |
50 |
4.9% |
| Pro[P] |
51 |
5.0% |
| Ser[S] |
80 |
7.9% |
| Thr[T] |
62 |
6.1% |
| Trp[W] |
17 |
1.7% |
| Tyr[Y] |
34 |
3.4% |
| Val[V] |
60 |
5.9% |
| Total Length |
1014 |
|
| Instability index (II) |
37.22 |
| This classifies the protein as stable |
|
| Carbon |
5139 |
| Hydrogen |
7953 |
| Nitrogen |
1403 |
| Oxygen |
1526 |
| Sulfur |
19 |
| Total number of atoms |
16040 |
| Formula = C5139H7953N1403O1526S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
144535 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
144160 M-1 cm-1 |
| assuming all Cys residues are reduced |
144160 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
144535 M-1 cm-1 |
|
|
| Codon Bias |
0.235 |
| Codon Adaptation Index |
0.233 |
| Frequency of Optimal Codons |
0.539 |
| Hydropathicity of Protein |
-0.485 |
| Aromaticity Score |
0.100 |
|
