RPI1/YIL119C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 8 2.0%
Arg[R] 9 2.2%
Asn[N] 65 16.0%
Asp[D] 14 3.4%
Cys[C] 4 1.0%
Gln[Q] 28 6.9%
Glu[E] 19 4.7%
Gly[G] 7 1.7%
His[H] 8 2.0%
Ile[I] 24 5.9%
Leu[L] 32 7.9%
Lys[K] 33 8.1%
Met[M] 10 2.5%
Phe[F] 20 4.9%
Pro[P] 22 5.4%
Ser[S] 57 14.0%
Thr[T] 21 5.2%
Trp[W] 3 0.7%
Tyr[Y] 12 2.9%
Val[V] 11 2.7%
Total Length 407

Instability index
Instability index (II) 48.70
This classifies the protein as unstable

Atomic composition
Carbon 2028
Hydrogen 3169
Nitrogen 579
Oxygen 657
Sulfur 14
Total number of atoms 6447
Formula = C2028H3169N579O657S14

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 34630 M-1 cm-1
assuming NO Cys residues appear as half cystines 34380 M-1 cm-1
assuming all Cys residues are reduced 34380 M-1 cm-1
assuming all pairs of Cys residues form cystines 34630 M-1 cm-1

Aliphatic index
Aliphatic index 63.46

Coding Region Translation Calculations
Codon Bias 0.015
Codon Adaptation Index 0.148
Frequency of Optimal Codons 0.437
Hydropathicity of Protein -0.927
Aromaticity Score 0.086