Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
89 |
6.1% |
| Arg[R] |
28 |
1.9% |
| Asn[N] |
88 |
6.0% |
| Asp[D] |
91 |
6.2% |
| Cys[C] |
6 |
0.4% |
| Gln[Q] |
58 |
4.0% |
| Glu[E] |
128 |
8.8% |
| Gly[G] |
81 |
5.5% |
| His[H] |
19 |
1.3% |
| Ile[I] |
62 |
4.2% |
| Leu[L] |
97 |
6.6% |
| Lys[K] |
113 |
7.7% |
| Met[M] |
18 |
1.2% |
| Phe[F] |
87 |
6.0% |
| Pro[P] |
75 |
5.1% |
| Ser[S] |
209 |
14.3% |
| Thr[T] |
102 |
7.0% |
| Trp[W] |
4 |
0.3% |
| Tyr[Y] |
20 |
1.4% |
| Val[V] |
85 |
5.8% |
| Total Length |
1460 |
|
| Instability index (II) |
48.69 |
| This classifies the protein as unstable |
|
| Carbon |
6939 |
| Hydrogen |
10851 |
| Nitrogen |
1845 |
| Oxygen |
2376 |
| Sulfur |
24 |
| Total number of atoms |
22035 |
| Formula = C6939H10851N1845O2376S24 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
52175 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
51800 M-1 cm-1 |
| assuming all Cys residues are reduced |
51800 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
52175 M-1 cm-1 |
|
|
| Codon Bias |
0.052 |
| Codon Adaptation Index |
0.143 |
| Frequency of Optimal Codons |
0.442 |
| Hydropathicity of Protein |
-0.595 |
| Aromaticity Score |
0.076 |
|
