COX5B/YIL111W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 9 6.0%
Arg[R] 9 6.0%
Asn[N] 9 6.0%
Asp[D] 4 2.6%
Cys[C] 0 0.0%
Gln[Q] 6 4.0%
Glu[E] 11 7.3%
Gly[G] 13 8.6%
His[H] 1 0.7%
Ile[I] 5 3.3%
Leu[L] 18 11.9%
Lys[K] 14 9.3%
Met[M] 3 2.0%
Phe[F] 5 3.3%
Pro[P] 8 5.3%
Ser[S] 8 5.3%
Thr[T] 12 7.9%
Trp[W] 6 4.0%
Tyr[Y] 4 2.6%
Val[V] 6 4.0%
Total Length 151

Instability index
Instability index (II) 27.90
This classifies the protein as stable

Atomic composition
Carbon 776
Hydrogen 1223
Nitrogen 215
Oxygen 221
Sulfur 3
Total number of atoms 2438
Formula = C776H1223N215O221S3

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 38960 M-1 cm-1

Aliphatic index
Aliphatic index 76.89

Coding Region Translation Calculations
Codon Bias 0.011
Codon Adaptation Index 0.104
Frequency of Optimal Codons 0.387
Hydropathicity of Protein -0.627
Aromaticity Score 0.099